CD95 (L315) polyclonal antibody

Product Specifications

Background

Cytotoxic T lymphocyte (CTL) -mediated cytotoxicity constitutes an important component of specific effector mechanisms in immuno- surveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FASbased mechanism involves the transducing molecule FAS (also designated APO-1) and its ligand (FAS-L) . The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating; surface antigen FAS; FASLG receptor; CD95; FAS; APT1; FAS1; TNFRSF6

Swiss Prot

P25445

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 280-330 of Human CD95.

Conjugation

Unconjugated

Related Pathways

Death Receptor Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 45 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD95 (L315) polyclonal antibody detects endogenous levels of CD95 protein.

Applications Notes

Western blot (WB) analysis of CD95 (L315) pAb at 1:500 dilution Lane1:Jurkat whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:SP2/0 whole cell lysate (40ug)