HYD (T14) polyclonal antibody
Product Specifications
Background
EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth. EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; Hyperplastic discs protein homolog; hHYD; Progestin-induced protein; UBR5; EDD; EDD1; HYD; KIAA0896
Swiss Prot
O95071
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 1-50 of Human HYD.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 309 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
HYD (T14) polyclonal antibody detects endogenous levels of HYD protein.
Applications Notes
Western blot (WB) analysis of HYD (T14) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:HK-2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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