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Ki-67 (V3242) polyclonal antibody

Product Specifications

Background

Ki-67 is a nuclear protein that is expressed in proliferating cells and may be required for maintaining cell proliferation. Ki-67 has been used as a marker for cell proliferation of solid tumors and some hematological malignancies. A correlation has been demonstrated between Ki-67 index and the histopathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Antigen KI-67; MKI67; Ki67; Ki 67

Swiss Prot

P46013

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids C-terminus of Human Ki-67.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 359 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ki-67 (V3242) polyclonal antibody detects endogenous levels of Ki-67 protein.

Applications Notes

Western blot (WB) analysis of Ki-67 (V3242) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug) Lane5:SK-OVCAR3 whole cell lysate (40ug) Lane6:DLD whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

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