Calnexin (D577) polyclonal antibody
Product Specifications
Background
Calnexin and Calregulin (also called Calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum—Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; CANX
Swiss Prot
P27824
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 540-590 of Human Calnexin.
Conjugation
Unconjugated
Applications
WB IHC IF IP
Dilution
WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Calnexin (D577) polyclonal antibody detects endogenous levels of Calnexin protein.
Applications Notes
Western blot (WB) analysis of Calnexin (D577) polyclonal antibody at 1:500 dilution Lane1:786-O whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:The kidney tissue lysate of Fetal Mouse (40ug) Lane5:The kidney tissue lysate of Fetal Rat (40ug)
Host or Source
Rabbit
Available Sizes
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