Caldesmon (S783) polyclonal antibody
Product Specifications
Background
Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular and non-muscular forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. Alternative splicing of the gene encoding Caldesmon results in five isoforms. Muscular Caldesmon (isoform 1), also designated high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon (isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon (L-CAD), is widely expressed in non-muscle tissues and cells.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Caldesmon; CDM; CALD1; CAD; CDM
Swiss Prot
Q05682
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 750-800 of Human Caldesmon.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
70-80 kDa non-muscle 120-150 kDa smooth muscle
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Caldesmon (S783) polyclonal antibody detects endogenous levels of Caldesmon protein.
Applications Notes
Western blot (WB) analysis of Caldesmon (S783) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (20ug) Lane2:Hela whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:MEF whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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