TH (Q25) polyclonal antibody

Product Specifications

Background

The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson’s disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH; TYH

Swiss Prot

P07101

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminus of Human TH.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas, Dopamine Signaling in Parkinson's Disease Plathway

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 58 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Tyrosine Hydroxylase (Q25) polyclonal antibody detects endogenous levels of Tyrosine Hydroxylase protein.

Applications Notes

Western blot (WB) analysis of TH (Q25) pAb at 1:2000 dilution Lane1:The Heart tissue lysate of Mouse (40ug) Lane2:The Heart tissue lysate of Rat (40ug)