Bad (I106) polyclonal antibody

Product Specifications

Background

Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

BAD, Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8

Swiss Prot

Q92934

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 72-126 of Human Bad.

Conjugation

Unconjugated

Related Pathways

PI3K AKT signaling, Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 24 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Bad (I106) polyclonal antibody detects endogenous levels of Bad protein

Applications Notes

Western blot (WB) analysis of Bad (R149) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:SP2/0 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)