Cyclin B1 (I120) polyclonal antibody
Product Specifications
Background
In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13 SUC1) . The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has estalished that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CCNB1; CCNB; cyclin B1; G2/mitotic-specific cyclin-B1
Swiss Prot
P14635
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 90-140 of Human Cyclin B1.
Conjugation
Unconjugated
Related Pathways
Cell Cycle G2 M DNA Damage Signaling Pathway
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cyclin B1 (I120) polyclonal antibody detects endogenous levels of Cyclin B1 protein.
Applications Notes
Western blot (WB) analysis of Cyclin B1 (I120) polyclonal antibody at 1:500 dilution Lane1:MCF-7whole cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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