ND1 polyclonal antibody

Product Specifications

Background

NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 1 (ND1) binds rotenone and rotenone analogs and might be involved in electron transfer to ubiquinone. Mutations in the ND1 gene may be implicated in several disorders, including Leber hereditary optic neuropathy, Alzheimer disease, and Parkinson disease.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

NADH-ubiquinone oxidoreductase chain 1 (EC:1.6.5.3) ; NADH dehydrogenase subunit 1; Mtnd1; mt-Nd1; Nd1

Swiss Prot

P03888

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to amino acids 189-202 of Mouse ND1.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 36 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ND1 polyclonal antibody detects endogenous levels of ND1 protein.

Applications Notes

Western blot (WB) analysis of ND1 polyclonal antibody at 1:1000 dilution Lane1:HEK293T whole cell lysate (57μg) Lane2:HepG-2 whole cell lysate (50μg) Lane3:H9C2 whole cell lysate (37μg) Lane4:NIH/3T3 whole cell lysate (39μg) Lane5:The muscle tissue lysate of Mouse (33μg) Lane6:The muscle tissue lysate of Rat (37μg)