TGase2 (D11) polyclonal antibody
Product Specifications
Background
Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE) . The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases) . TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
TGM2; Protein-glutamine gamma-glutamyltransferase 2; Tissue transglutaminase; Transglutaminase C; TG (C) ; TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2
Swiss Prot
P21980
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to the N-terminus of Human TGase2.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 77 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Transglutaminase 2 (D11) polyclonal antibody detects endogenous levels of Transglutaminase 2 protein.
Applications Notes
Western blot (WB) analysis of TGase2 (D11) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug) Lane6:HCT116 whole cell lysate (40ug) Lane7:L02 whole cell lysate (20ug)
Host or Source
Rabbit
Available Sizes
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