Synuclein-pan (A19) polyclonal antibody

Product Specifications

Background

The synuclein family members, including α-synuclein (also designated NACP for non-β amyloid component) and β-synuclein, are predominantly expressed in the brain and are speculated to be involved in synaptic regulation and neuronal plasticity. α-synuclein is localized to neuronal cell bodies and synapses. α-synuclein was first identified as a component of Alzheimer’s disease amyloid plaques. Abnormal platelet function in Alzheimer’s disease has been demonstrated. During megakaryocytic differentiation α-synuclein was found to be up-regulated, while β-synuclein is down-regulated, indicating that coordinate expression of synucleins may be important during hematopoetic cell differentiation. A mutant form of α-synuclein has been found in patients with early onset Parkinson’s disease.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Alpha-synuclein; α-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; NACP; PARK1; Beta-synuclein; SNCB; β-synuclein; synuclein-β; Gamma-synuclein; γ-synuclein; synuclein-γ; Breast cancer-specific gene 1 protein; Persyn; Synoretin; SR; SNCG; BCSG1; PERSYN; PRSN

Swiss Prot

P37840/O76070/Q16143

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids N-terminus of 19-30 Synuclein-α.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 18 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Synuclein-pan (A19) polyclonal antibody detects endogenous levels of Synuclein-pan (α-synuclein, β-synuclein, γ-synuclein) protein.

Applications Notes

Western blot (WB) analysis of Synuclein (A19) pAb at 1:500 dilution Lane1:Panc1 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:The Brain tissue lysate of Mouse (40ug) Lane5:The Brain tissue lysate of Rat (40ug)