Synaptotagmin (M303) polyclonal antibody

Product Specifications

Background

Synaptotagmins are a large gene family of synaptic vesicle type III integral membrane proteins that function as regulators of both exocytosis and endocytosis and are involved in neurotransmitter secretion from small secretory vesicles. Calcium binds to Synaptotagmin I which triggers neurotransmitter release at the synapse. Synaptotagmin II is phosphorylated by WNK1 in a process that regulates calcium-dependent interactions. Synaptotagmin III is involved in calcium-dependent exocytosis of secretory vesicles in endocrine cells and neurons. Synaptotagmin IV is expressed in neuronal tissues, and has the highest mRNA levels in the hippocampus.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Synaptotagmin-1; Synaptotagmin 1; Synaptotagmin I; SytI; SYT1; SVP65; SYT

Swiss Prot

P21579

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 280-330 of Human Synaptotagmin.

Conjugation

Unconjugated

Related Pathways

Vesicle Trafficking presynaptic signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 64 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Synaptotagmin (M303) polyclonal antibody detects endogenous levels of Synaptotagmin protein.

Applications Notes

Western blot (WB) analysis of Synaptotagmin (M303) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate