Rit1 (W204) polyclonal antibody
Product Specifications
Background
ROC 1 and ROC 2 are the two highly conserved RING finger proteins that are homologous to APC 11, a subunit of the anaphase promoting complex (APC) . ROC 1 and ROC 2 commonly interact with all cullins while APC 11 specifically interacts with APC 2, a cullin related APC subunit. ROC 1 and APC 11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1 and E2 dependent manner. ROC 1 is thought to play a unique role in the ubiquitination reaction by heterodimerizing with cullin 1 to catalyze ubiquitin polymerization.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
GTP-binding protein Rit1; Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1; RIT1; RIBB; RIT; ROC1
Swiss Prot
Q92963
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 170-220 of Human Rit1.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 25 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ROC1 (W204) polyclonal antibody detects endogenous levels of ROC1 protein.
Applications Notes
Western blot (WB) analysis of Rit1 (W204) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:H9C2 whole cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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