PAR4 (D313) polyclonal antibody
Product Specifications
Background
Aberrant regulation of any of these cell processes can result in cancer. Cell death during embryogenesis, tissue atrophy and normal tissue turnover is called apoptosis and is characterized by cytoplasmic and nuclear condensation, nuclear disorganization and fragmentation of genomic DNA into 180-200 base pair oligomers. Five ionomycin-inducible complementary cDNAs, designated PAR1, 2, 3, 4 and 5, have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of MKP-1, PAR2 as the injury-inducible gene HB-EGF, and PAR3 as the serum-induced gene Cyr61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 (prostate apoptosis response 4) is specifically expressed by cells entering apoptosis and is not induced during growth factor stimulation, oxidative stress, necrosis or growth arrest. The PAR4 gene encodes a protein with a putative nuclear localization signal and carboxy terminal leucine zipper.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Par-4; PAWR
Swiss Prot
Q96IZ0
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 283-332 of Human PAR4.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 45 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Prostate Apoptosis Response protein-4 (D313) polyclonal antibody detects endogenous levels of Prostate Apoptosis Response protein-4 protein.
Applications Notes
Western blot (WB) analysis of PAR4 (D313) polyclonal antibody at 1:500 dillution Lane1:U-87MG whole cell lysate (40μg) Lane2:HCT-116 whole cell lysate (40μg) Lane3:786-O whole cell lysate (40μg) Lane4:The Testis tissue lysate of Mouse (40μg) Lane5:The Testis tissue lysate of Rat (40μg)
Host or Source
Rabbit
Available Sizes
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