PML (P39) polyclonal antibody
Product Specifications
Background
The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t (15;17) (q22; q11.2-q12) chromosomal trans-location of the retinoic acid receptor α (RARα) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARα chimeric protein. Myeloid precursor cells expressing the PML/ RARα chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARα transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR) -histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARα also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Protein PML; Promyelocytic leukemia protein; RING finger protein 71; Tripartite motif-containing protein 19; MYL; PP8675; RNF71; TRIM19
Swiss Prot
P29590
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to the N-terminal of Human PML.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 98 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PML (P39) polyclonal antibody detects endogenous levels of PML protein.
Applications Notes
Western blot (WB) analysis of PML (P39) pAb at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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