PDPK1 (K235) polyclonal antibody

Product Specifications

Background

PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

3-phosphoinositide-dependent protein kinase 1; hPDK1; PDPK1; PDK1

Swiss Prot

O15530

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 211-260 of Human PDPK1.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, PI3K AKT signaling, Protein Kinase C Signaling, Translational Contral elF4 and p70 S6 Kinase, Inhibition of Apoptosis, Insulin &Glucose Signaling, MTOR signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 63 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PDPK1 (K235) polyclonal antibody detects endogenous levels of PDPK1 protein.

Applications Notes

Western blot (WB) analysis of PDPK1 (K235) Antibody at 1:1000 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug)