Patched (A2) polyclonal antibody
Product Specifications
Background
PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH) . PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Protein patched homolog 1; PTC; PTC1; PTCH1; PTCH
Swiss Prot
Q13635
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Patched.
Conjugation
Unconjugated
Related Pathways
Hedgehog Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 160 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Patched (A2) polyclonal antibody detects endogenous levels of Patched protein.
Applications Notes
Western blot (WB) analysis of Patched (A2) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:The Testis tissue lysate of Mouse (40ug) Lane6:The Testis tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
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