P18 INK4c (D142) polyclonal antibody

Product Specifications

Background

The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein p16. Isolated members of the p16 family have been designated p15 and p18, respectively. The p16 related protein, p18, interacts strongly with Cdk6 and to a lesser extent with Cdk4, but lacks apparent interaction with other Cdks. Recombinant p18 has been shown to inhibit cyclin D-Cdk6 kinase activity. In contrast to p21/p27 that form ternary complexes with cyclin-Cdks, only binary complexes of p15, p16 and p18 have been identified in association with Cdk4 and/or Cdk6.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; p18-INK4c; p18-INK6; CDKN2C; CDKN6; p18INK4c; p18INK6; p18 INK6

Swiss Prot

P42773

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 100-150 of Human p18 INK4c.

Conjugation

Unconjugated

Related Pathways

Cell Cycle Control G1 S Checkpoint

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 18 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P18 INK4c (D142) polyclonal antibody detects endogenous levels of p18 INK4c protein.

Applications Notes

Western blot (WB) analysis of p18 INK4c (D142) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)