MEK-3 (S183) polyclonal antibody

Product Specifications

Background

The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinaseegulatory threonine and tyrosine residues present in thehr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6hosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAPKK 3; MAPK/ERK kinase 3; MEK 3; Stress-activated protein kinase kinase 2; SAPK kinase 2; SAPKK-2; SAPKK2; MAP2K3; MEK3; MKK3; PRKMK3; SKK2

Swiss Prot

P46734

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 150-200 of Human MEK-3.

Conjugation

Unconjugated

Related Pathways

Signaling Pathways Activating p38 MAP Kinase

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 39 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MEK-3 (S183) polyclonal antibody detects endogenous levels of MEK-3 protein.

Applications Notes

Western blot (WB) analysis of MEK-3 (S183) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:A375 whole cell lysate (40ug) Lane3:The Kidney tissue lysate of Mouse (40ug) Lane4:The Testis tissue lysate of Rat (40ug)