MCM5 (F51) polyclonal antibody

Product Specifications

Background

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2–6) during apoptosis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46; MCM5; CDC46

Swiss Prot

P33992

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 30-80 of Human MCM5.

Conjugation

Unconjugated

Applications

WB IHC IF IP

Dilution

WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 82 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MCM5 (F51) polyclonal antibody detects endogenous levels of MCM5 protein.

Applications Notes

Western blot (WB) analysis of MCM5 (F51) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)