Cytokeratin 7 (L451) polyclonal antibody

Product Specifications

Background

Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cytokeratin-7; CK-7; Cytokeratin 7; Cytokeratin7; Keratin 7; Keratin7; Keratin-7; KRT7; SCL; Sarcolectin; Type-II keratin Kb7

Swiss Prot

P08729

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 411-460 of Human Cytokeratin 7.

Conjugation

Unconjugated

Related Pathways

Contribution of Soluble Factors to EMT

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 51 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cytokeratin 7 (L451) polyclonal antibody detects endogenous levels of Cytokeratin 7 protein.

Applications Notes

Western blot (WB) analysis of Cytokeratin 7 (L451) polyclonal antibody at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:A431 whole cell lysate (10ug) Lane3:PC12 whole cell lysate (40ug) Lane4:SP2/0 whole cell lysate (40ug)