Cytokeratin 5 (S573) polyclonal antibody

Product Specifications

Background

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB) .

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Keratin, type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; Cytokeratin5; Short name=CK-5; CK5; Keratin-5; Keratin5; Keratin 5; Short name=K5; Type-II keratin Kb5; KRT5

Swiss Prot

P13647

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 550-600 of Human Cytokeratin 5.

Conjugation

Unconjugated

Related Pathways

Contribution of Soluble Factors to EMT

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 62 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cytokeratin 5 (S573) polyclonal antibody detects endogenous levels of Cytokeratin 5 protein.

Applications Notes

Western blot (WB) analysis of Cytokeratin 5 (S573) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:NIH-3T3 whole cell lysate (40ug)