Cytokeratin 18 (P27) polyclonal antibody

Product Specifications

Background

Cytokeratin 18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Involved in the uptake of thrombin-antithrombin complexes by hepatic cells By similarity. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6) -mediated barrier protection.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; Short name=CK-18; Keratin-18; Short name=K18; KRT18; CYK18; PIG46; KRT 18; CK 18; CK18; Keratin 18; Keratin18; CK-18

Swiss Prot

P05783

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 1-50 of Human Cytokeratin 18.

Conjugation

Unconjugated

Related Pathways

Contribution of Soluble Factors to EMT

Applications

WB IHC IF IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 46 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cytokeratin 18 (P27) polyclonal antibody detects endogenous levels of Cytokeratin 18 protein.

Applications Notes

Western blot (WB) analysis of Cytokeratin 18 (P27) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate