Cytokeratin 16 (S451) polyclonal antibody
Product Specifications
Background
Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins) . Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Keratin, type I cytoskeletal 16; Cytokeratin-16; Cytokeratin16; CK-16; CK16; Keratin-16; Keratin 16; Keratin16; Short name=K16; KRT16; KRT16A
Swiss Prot
P08779
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 421-470 of Human Cytokeratin 16.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 52 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cytokeratin 16 (S451) polyclonal antibody detects endogenous levels of Cytokeratin 16 protein.
Applications Notes
Western blot (WB) analysis of Cytokeratin 16 (S451) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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