Cytokeratin 16 (S451) polyclonal antibody

Product Specifications

Background

Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins) . Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Keratin, type I cytoskeletal 16; Cytokeratin-16; Cytokeratin16; CK-16; CK16; Keratin-16; Keratin 16; Keratin16; Short name=K16; KRT16; KRT16A

Swiss Prot

P08779

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 421-470 of Human Cytokeratin 16.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 52 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cytokeratin 16 (S451) polyclonal antibody detects endogenous levels of Cytokeratin 16 protein.

Applications Notes

Western blot (WB) analysis of Cytokeratin 16 (S451) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate