PRAF2 (L166) polyclonal antibody

Product Specifications

Background

JM4 (Jena-Muenchen 4), also known as PRAF2 (PRA1 domain family, member 2), is a 178 amino acid endosomal multi-pass membrane protein involved in vesicular trafficking and Endoplasmic reticulum/Golgi transport. As a member of the PRA1 family, JM4 contains four putative transmembrane (TM) domains, interacts with the CC chemokine receptor 5 (CCR5) and colocalizes with Calnexin in the ER and mannose 6-phosphate receptor (CD-MPR) in the Golgi apparatus. While ubiquitously expressed, JM4 has been found at high levels in small intestine, lung, pancreas, spleen, Purkinje cells of the cerebellum and in neuronal cells of the hippocampus, cerebral cortex and lateral ventricles of the brain. JM4 plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis and has been implicated in human cancer.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

PRAF2; JM4; PRA1 family protein 2

Swiss Prot

O60831

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 141-190 of Human PRAF2.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 20 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

JM4/PRAF2 (L166) polyclonal antibody detects endogenous levels of JM4/PRAF2 protein.

Applications Notes

Western blot (WB) analysis of PRAF2 (L166) polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Mouse (40ug) Lane2:The Brain tissue lysate of Rat (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)