IKB-ε (D17) polyclonal antibody
Product Specifications
Background
IkB-ε (I-kappa-B-epsilon), also known as NFKBIE (NFκB inhibitor epsilon) or IKBE, is a 500 amino acid protein that belongs to the NFκB inhibitor family. Localizing to the cytoplasm, IkB-ε is highly expressed in spleen, testis and lung, with lower levels of expression found in kidney, pancreas, heart, placenta, brain, granulocytes and macrophages. IkB-ε inhibits c-Rel and NFκB subunits p50, p52 and p65 by forming a complex with them in the cytoplasm, preventing them from activating genes in the nucleus. IkB-ε undergoes serine phosphorylation, resulting in the protein being marked for destruction via the ubiquitination pathway.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon; NFKBIE; IKBE; IkB ε; IkBε
Swiss Prot
O00221
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 1-50 of Human IKB-ε.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 45 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IκB-ε (D17) polyclonal antibody detects endogenous levels of IκB-ε protein.
Applications Notes
Western blot (WB) analysis of IkB-ε (D17) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:NIH-3T3 cell lysate Lane3:PC12 cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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