IGF-1R (G1155) polyclonal antibody

Product Specifications

Background

Insulin-like growth factor-I receptor (IGF-IR) is an RTK that stimulates growth in many different cell types, blocks apoptosis, acts as an intermediate of many growth hormone responses and may stimulate the growth of some types of cancer. The IGF-IR cognate ligand insulin-like growth factor-I (IGF-I) promotes association of IGF-IR with Shc, GRB2 and Sos 1, which initiates Ras and ERK kinase cascades, thereby modifying transcription factor activity, such as activation of the Elk transcription factors. The modular phosphotyrosine binding (PTB) domains of insulin receptor substrate (IRS) -1 and -2 can associate with active IGF-IR and initiate phosphatidylinositol 3-kinase-dependent downstream signals.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD221; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; IGF1R

Swiss Prot

P08069/P06213

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 1121-1170 of Human IGF-1R.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis, Insulin &Glucose Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 95,155 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IGF-1R (G1155) polyclonal antibody detects endogenous levels of IGF-1R protein. This antibody may cross-reacts with insulin receptor protein.

Applications Notes

Western blot (WB) analysis of IGF-1R (G1155) pAb at 1:500 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:Panc1 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)