HSP90B (K248) polyclonal antibody
Product Specifications
Background
HSP 90 family members guide the normal folding, intracellular disposition and proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90alpha, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90beta, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC epsilon to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84; HSP90AB1; HSP90B; HSPC2; HSPCB
Swiss Prot
P08238
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 211-260 of Human HSP90B.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics, Hypoxia Signaling
Applications
WB IHC IF IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
HSP90B (K248) polyclonal antibody detects endogenous levels of HSP90B protein.
Applications Notes
Western blot (WB) analysis of HSP90B (K248) polyclonal antibody at 1:500 dilution Lane1:The Lung tissue lysate of Rat (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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