HDAC7 (G932) polyclonal antibody

Product Specifications

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC7 is a histone deacetylase that interacts with the adaptor mSin3A. The interaction of HDAC7 with mSin3A suggests the association of multiple repression complexes of transcription factors.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone deacetylase 7; HDAC 7; HDAC-7

Swiss Prot

Q8WUI4

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 901-950 of Human HDAC7.

Conjugation

Unconjugated

Related Pathways

Notch Signaling, Cell Cycle Control G1 S Checkpoint, Epigenetic Writers and Erasers of Histone H3, Epigenetic Writers and Erasers of Histones H2A H2B and H4

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 105 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HDAC7 (G932) polyclonal antibody detects endogenous levels of HDAC7 protein.

Applications Notes

Western blot (WB) analysis of HDAC7 (G932) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:3T3-L1 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:U-87MG whole cell lysate (40ug)