HDAC5 (E1106) polyclonal antibody

Product Specifications

Background

HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone deacetylase 5; Short name=HD5; Antigen NY-CO-9; HDAC-5; KIAA0600

Swiss Prot

Q9UQL6

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the C-terminus of Human HDAC5.

Conjugation

Unconjugated

Related Pathways

Notch Signaling, Nuclear Receptor Signaling, Cell Cycle Control G1 S Checkpoint, Epigenetic Writers and Erasers of Histone H3, Epigenetic Writers and Erasers of Histones H2A H2B and H4

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 121 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HDAC5 (E1106) polyclonal antibody detects endogenous levels of HDAC5 protein.

Applications Notes

Western blot (WB) analysis of HDAC5 (E1106) polyclonal antibody at 1:500 dilution Lane1:H9C2 whole cell lysate (40ug) Lane2:NIH-3T3 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug)