PARK7 (P54) polyclonal antibody

Product Specifications

Background

The DJ-1 gene encodes a highly-conserved protein which is implicated in a number of cell processes. DJ-1 was first identified as a novel oncogene that transformed mouse NIH/3T3 cells in cooperation with activated Ras. Additionally, DJ-1 was cloned in rat as SP22 or CAP-1 and found to be an infertilityrelated sperm protein, whose expression is reduced in sperm treated with toxicants. DJ-1 also positively regulates the androgen receptor (AR) by forming a complex with PIASxα, a negative regulator of AR. The gene encoding human DJ-1 maps to chromosome 1p36.33-p36.12, a region identified as a hot spot of chromosome abnormalities in several tumor cells. Subsequently, mutations in the DJ-1 gene have been implicated in Parkinson’s disease, and loss of DJ-1 function leads to neurodegeneration. DJ-1 is an ubiquitously expressed protein that is induced in response to growth stimuli and translocates from the cytoplasm to the nucleus during the S phase of the cell cycle.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Protein DJ-1; Oncogene DJ1; Parkinson disease protein 7; PARK7

Swiss Prot

Q99497

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 22-70 of Human PARK7.

Conjugation

Unconjugated

Related Pathways

Dopamine Signaling in Parkinson's Disease Plathway

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 22 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PARK7 (P54) polyclonal antibody detects endogenous levels of PARK7 protein.

Applications Notes

Western blot (WB) analysis of PARK7 (P54) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (20ug) Lane2:HepG2 whole cell lysate (20ug) Lane3:LO2 whole cell lysate (20ug) Lane4:The testis tissue lysate of Mouse (40ug) Lane5:The testis tissue lysate of Rat (40ug)