Cyclin E1 (I119) polyclonal antibody

Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of Cyclin E1 (I119) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (20ug) Lane2:SK-OVCAR3 whole cell lysate (20ug) Lane3:Hela whole cell lysate (20ug) Lane4:PC12 whole cell lysate (20ug) Lane5:CT26 whole cell lysate (20ug) Lane6:The Testis tissue lysate of Rat (20ug)