Cyclin E1 (I119) polyclonal antibody
Product Specifications
Background
Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
G1/S-specific cyclin-E1; CCNE1; CCNE
Swiss Prot
P24864
Reactivity
Human, Mouse
Immunogen
Synthetic peptide, corresponding to amino acids 90-135 of Human Cyclin E1.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 49 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cyclin E1 (I119) polyclonal antibody detects endogenous levels of Cyclin E1 protein.
Applications Notes
Western blot (WB) analysis of Cyclin E1 (I119) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (20ug) Lane2:SK-OVCAR3 whole cell lysate (20ug) Lane3:Hela whole cell lysate (20ug) Lane4:PC12 whole cell lysate (20ug) Lane5:CT26 whole cell lysate (20ug) Lane6:The Testis tissue lysate of Rat (20ug)
Host or Source
Rabbit
Available Sizes
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