CUL1 (Y761) polyclonal antibody

Product Specifications

Background

CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CUL 1; CUL-1; CUL1; CUL1; Cullin-1; Cullin1; MGC149834; MGC149835

Swiss Prot

Q13616

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 728-780 of Human CUL-1.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 90 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cullin 1 (Y761) polyclonal antibody detects endogenous levels of Cullin 1 protein.

Applications Notes

Western blot (WB) analysis of CUL1 (Y761) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Rat (40ug) Lane2:The Lung tissue lysate of Rat (40ug) Lane3:The Lung tissue lysate of Mouse (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:HCC827 whole cell lysate (40ug)