CUL1 (Y761) polyclonal antibody
Product Specifications
Background
CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CUL 1; CUL-1; CUL1; CUL1; Cullin-1; Cullin1; MGC149834; MGC149835
Swiss Prot
Q13616
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 728-780 of Human CUL-1.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cullin 1 (Y761) polyclonal antibody detects endogenous levels of Cullin 1 protein.
Applications Notes
Western blot (WB) analysis of CUL1 (Y761) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Rat (40ug) Lane2:The Lung tissue lysate of Rat (40ug) Lane3:The Lung tissue lysate of Mouse (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:HCC827 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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