CREB (I127) polyclonal antibody

Product Specifications

Background

Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1

Swiss Prot

P16220

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 100-150 of Human CREB.

Conjugation

Unconjugated

Related Pathways

Signaling Pathways Activating p38 MAP Kinase

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CREB (I127) polyclonal antibody detects endogenous levels of CREB protein.

Applications Notes

Western blot (WB) analysis of CREB (I127) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)