CREB (I127) polyclonal antibody
Product Specifications
Background
Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1
Swiss Prot
P16220
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 100-150 of Human CREB.
Conjugation
Unconjugated
Related Pathways
Signaling Pathways Activating p38 MAP Kinase
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CREB (I127) polyclonal antibody detects endogenous levels of CREB protein.
Applications Notes
Western blot (WB) analysis of CREB (I127) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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