Connexin 43 (S369) polyclonal antibody
Product Specifications
Background
Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Gap junction alpha-1 protein; Connexin-43; Connexin 43; Connexin43; Cx43; Cx-43; Gap junction 43 kDa heart protein; GJA1; GJAL
Swiss Prot
P17302
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 340-390 of Human Connexin 43.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 43 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Connexin 43 (S369) polyclonal antibody detects endogenous levels of Connexin 43 protein.
Applications Notes
Western blot (WB) analysis of Connexin 43 (S369) polyclonal antibody at 1:500 dilution Lane1:MEF whole cell lysate (20ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:COS-7 whole cell lysate (20ug) Lane4:HEK239T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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