Claudin-4 (P192) polyclonal antibody

Product Specifications

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM) . Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Claudin 4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein; CLDN4; CPER; CPETR1; WBSCR8

Swiss Prot

O14493

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-4.

Conjugation

Unconjugated

Applications

WB IHC IF IP

Dilution

WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 22 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Claudin 4 (P192) polyclonal antibody detects endogenous levels of Claudin 4 protein.

Applications Notes

Western blot (WB) analysis of Claudin-4 (P192) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:DLD whole cell lysate (40ug)