Cdk2 (V154) polyclonal antibody
Product Specifications
Background
Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) . Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase; CDK2; CDKN2
Swiss Prot
P24941
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 125-170 of Human Cdk2.
Conjugation
Unconjugated
Related Pathways
Cell Cycle Control G1 S Checkpoint, Cell Cycle G2 M DNA Damage Signaling Pathway
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 34 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cdk2 (V154) polyclonal antibody detects endogenous levels of Cdk2 protein. Based on sequence similarity, the antibody may cross-react with Cdc2 protein.
Applications Notes
Western blot (WB) analysis of Cdk2 (V154) pAb at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:BV2 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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