Catenin-γ (731) polyclonal antibody

Product Specifications

Background

The catenins, α, β and γ, are proteins which bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherincomplexes play an important role mediating cellular adhesion. α-catenin was initially described as an E-cadherin associated protein, and since has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. β-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. β-catenin has also been found in complexes with the tumor suppressor protein APC. γ-catenin, also known as plakoglobin, binds with α-catenin and N-cadherin. It has been shown that the transmembrane phosphatase PTPμ associates with catenin/cadherin complexes and may regulate complex signaling.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Junction plakoglobin; Catenin gamma; Desmoplakin-3; CTNNG; DP3, JUP

Swiss Prot

P14923

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 700-750 of Human Catenin-γ.

Conjugation

Unconjugated

Related Pathways

Contribution of ECM and Cytoskeletal Factors to EMT, Adherens Junction signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 82 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Catenin-γ (731) polyclonal antibody detects endogenous levels of Catenin-γ protein.

Applications Notes

Western blot (WB) analysis of Catenin-γ (731) polyclonal antibody at 1:500 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:H1792 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)