BIK (M27) polyclonal antibody

Product Specifications

Background

Bik/Nbk (Bcl-2-interacting killer/natural born killer) is a potent pro-apoptotic protein belonging to a group of Bcl-2 family members that includes Bad, Bid, Bim, Hrk, and Noxa, containing a BH3 domain but lacking other conserved domains, BH1 or BH2. Functionally, Bik is able to bind to and antagonize anti-apoptotic Bcl-2 family members including Bcl-2, Bcl-xL, and viral homologs E1B-19K and EBV-BHFR1. The BH3 domain of Bik is essential for its apoptotic activity and interaction with survival proteins. Phosphorylation of Bik is correlated with an increase in its pro-apoptotic activity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4; BIK; NBK

Swiss Prot

Q13323

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminal of Human BIK.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 23 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

BIK (M27) pAb detects endogenous levels of BIK protein

Applications Notes

Western blot (WB) analysis of BIK (M27) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:RAW264.7 whole cell lysate (40ug)