PD1 monoclonal antibody (Humanized)

Product Specifications

Background

The humanized monoclonal anti-PD1 antibody can effectively block the combination between PDL1 and PD1, and will not cause HAMA reaction (anti mouse antibody response) . Programmed cell death protein 1 (PD-1) (UniProt Q15116), is a type I single transmembrane protein encoded by the PDCD1 gene in human. T-cell activation is generally self-limited as activated T cells express receptors such as PD-1. Maintenance of peripheral tolerance by preventing the development of autoimmunity due to T lymphocytes specific for self-antigens is essential for homeostasis of the immune system. PD-1 can bind two different but related ligands, PDL-1 and PDL-2.PD-1 receptor and its ligands PD-L1 and PD-L2play a vital role in the induction and maintenance of peripheral tolerance. The humanized monoclonal anti-PD1 antibody has demonstrated durable antitumor activity.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 50% Glycerol.

Product Name Alternative

Programmed cell death protein 1; Protein PD-1; hPD-1; CD279; PDCD1; PD1

Swiss Prot

Q15116

Reactivity

Human

Immunogen

Variable region genes (including VH &VL) of this antibody were derived from a single B cell by PCR.

Conjugation

Unconjugated

Related Pathways

Immune checkpoint signaling in the Tumor Microenvironment, Tumor-Infiltrating Immune Cell Marker Guide, T cell receptor signaling

Applications

Neutralization/Block

Dilution

Neutralization/Block

Purity

Recombinant IgG antibodies were expressed and secreted by 293F cells and purified by Protein A.

Modification

Unmodification

Molecular Weight

~ 32 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PD1 monoclonal antibody (Humanized) detects endogenous levels of human PD1 protein. Variable region genes (including VH &VL) of this antibody were derived from a single B cell by PCR.

Applications Notes

Western blot shows binding reaction of PD1 monoclonal antibody (Humanized) and recombinant human PD1 (with Fc tag) . Recombinant human PD1 (Fc) was electrophoresed by SDS-PAGE and transferred on PVDF membrane. PVDF membrane was probed with PD1 monoclonal antibody (Humanized) followed by HRP-conjugated Anti-Human IgG Fab secondary Antibody.