ATG5 polyclonal antibody
Product Specifications
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents . Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer . The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles . This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
APG5-like; Apoptosis-specific protein
Swiss Prot
Q9H1Y0
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full length Human ATG5.
Conjugation
Unconjugated
Related Pathways
Autophagy Signaling
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Modification
Unmodification
Molecular Weight
~ 55 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ATG5 polyclonal antibody detects endogenous levels of ATG5 protein.
Applications Notes
Western blot (WB) analysis of ATG 5 polyclonal antibody at 1:1000 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (30ug) Lane3:SK-OVCAR3 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:The brain tissue lysate of Mouse (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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