CAPG polyclonal antibody

Product Specifications

Background

Caldesmon, Filamin 1, Nebulin, Plastin, ADF, Gelsolin, CapG, Dematin and Cofilin are differentially expressed Actin-binding proteins. Both muscular (CDh) and non-muscular (CD1) forms of Caldesmon bind to Actin as well as to Calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CD1 is widely expressed in non-muscle tissues and cells. CapG, also designated Actin-regulatory protein and macrophage-capping protein, is a macrophage-specific protein that reversibly blocks the barbed ends of Actin filaments, but does not sever preformed ones. The interactions of CapG with Actin may be important in the regulation of nuclear and cytoplasmic structures. CapG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Macrophage-capping protein; Actin regulatory protein CAP-G; CAPG; AFCP, MCP

Swiss Prot

P40121

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fragment corresponding to a region within amino acids 62-349 of Human CAPG.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 43 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CAPG polyclonal antibody detects endogenous levels of CAPG protein.

Applications Notes

Western blot (WB) analysis of CAPG pAb at 1:500 dilution Lane1:The Lung tissue lysate of Mouse (40ug) Lane2:The Lung tissue lysate of Rat (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:H1792 whole cell lysate (40ug) Lane5:A375 whole cell lysate (40ug)