DHX36 polyclonal antibody

Product Specifications

Background

DDX36 (DEAH box protein 36), also known as MLE-like protein 1 and RNA helicase associated with AU-rich element ARE, is a 1,008 amino acid protein that is expressed in testis and may function in sex development and spermatogenesis.DDX36 plays a role in degradation and deadenylation of mRNAs that contain the consensus ARE sequence element in their 3'-UTR. There are three isoforms of DDX36 that exist as a result of alternative splicing events.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ATP-dependent RNA helicase DHX36; DEAH box protein 36; G4-resolvase 1; G4R1; MLE-like protein 1; RNA helicase associated with AU-rich element ARE; DHX36; DDX36; KIAA1488; MLEL1; RHAU

Swiss Prot

Q9H2U1

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length Human DHX36.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 115 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

DHX36 polyclonal antibody detects endogenous levels of DHX36 protein.

Applications Notes

Western blot (WB) analysis of DHX36 polyclonal antibody at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:L02 whole cell lysate (20ug) Lane3:HEK293T whole cell lysate (20ug) Lane4:The liver tissue lysate of Mouse (40ug) Lane5:The liver tissue lysate of Rat (40ug)