Lamin B1 polyclonal antibody
Product Specifications
Background
An important part of the nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of alpha helices and a carboxy terminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Lamin-B1; LMNB1; LMN2; LMNB
Swiss Prot
P20700
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full length Human Lamin B1.
Conjugation
Unconjugated
Applications
WB IHC IF IP
Dilution
WB: 1:5000~1:20000 IHC/IF: 1:100~1:1000 IP: 1:100~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 68 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Lamin B1 polyclonal antibody detects endogenous levels of Lamin B1 protein.
Applications Notes
Western blot (WB) analysis of Lamin B1 polyclonal antibody at 1:20000 dilution Lane1:A549 whole cell lysate (40ug) Lane2:DLD whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Mouse (40ug) Lane4:The Lung tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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