ULK1 (phospho-Ser636) polyclonal antibody
Product Specifications
Background
ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species conserved carboxyl-terminal domain. Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively. ULK1 and ULK2 are thought to auto-phosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; ATG1; Unc-51-like kinase 1; ULK1; KIAA0722
Swiss Prot
O75385
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human ULK1 around the phosphorylation site of Ser638.
Conjugation
Unconjugated
Related Pathways
PI3K AKT signaling, Autophagy Signaling, MTOR signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 150 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ULK1 (phospho-Ser638) polyclonal antibody detects endogenous levels of ULK1 protein only when phosphorylated at Ser638.
Applications Notes
Western blot (WB) analysis of ULK1 (Phospho-Ser638) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with PBS (1xPBS, PH7.4) for 1 hour whole cell lysate Lane3:HEK293T treated with Etoposide (20ng/ml) for 6 hours whole cell lysate Lane4:HEK293T whole cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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