LC3 (T50) polyclonal antibody
Product Specifications
Background
Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; LC3-I; LC3-II; LC3I; LC3II
Swiss Prot
Q9GZQ8/Q9H492/Q9BXW4
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human LC3.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, Autophagy Signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 15 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
LC3 (T50) polyclonal antibody detects endogenous levels of LC3-I and LC3-II protein.
Applications Notes
Western blot (WB) analysis of LC3 (T50) polyclonal antibody at 1:500 dilution Lane1:BV2 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (10ug) Lane4:L02 whole cell lysate (10ug) Lane5:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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