LC3 (T50) polyclonal antibody

Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of LC3 (T50) polyclonal antibody at 1:500 dilution Lane1:BV2 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (10ug) Lane4:L02 whole cell lysate (10ug) Lane5:HEK293T whole cell lysate (40ug)